Izumo1^em1Nino
Endonuclease-mediated Allele Detail

Summary

• Symbol: Izumo1^em1Nino
• Name: izumo sperm-egg fusion 1; endonuclease-mediated mutation 1, Naokazu Inoue
• MGI ID: MGI:6511748
• Synonyms: Izumo1-KO, IZUMO1_v1 KO
• Gene: Izumo1 Location: Chr7:45270251-45276653 bp, + strand Genetic Position: Chr7, 29.39 cM, cytoband B2
• Alliance: Izumo1^em1Nino page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing technology was used to generate a nine-base deletion adjacent to the initial IZUMO1_v1 methionine start codon ( IZUMO1_v1 (p.Met1del) / IZUMO1_v2 (p.Ala30-Met32del). A reduced amount of encoded protein is expressed via an alternative splice codon (IZUMO1_v2). (J:275429)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Izumo1 Mutation: 24 strains or lines available

References

• Original: J:275429 Saito T, et al., Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice. Sci Rep. 2019 Feb 28;9(1):3151
• All: 2 reference(s)