Ddx4^{em1(cre/ERT2)Utr}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ddx4^{em1(cre/ERT2)Utr}
• Name: DEAD box helicase 4; endonuclease-mediated mutation 1, Ken-ichi Yagami
• MGI ID: MGI:6509979
• Synonyms: Ddx4^CreERT2
• Gene: Ddx4 Location: Chr13:112734867-112789009 bp, - strand Genetic Position: Chr13, 63.87 cM
• Alliance: Ddx4^{em1(cre/ERT2)Utr} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, No functional change, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Ddx4^{em1(cre/ERT2)Utr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ddx4 (MGI:102670)

• Mutation details: A P2ACreERT2rGpA gene cassette was knockedin frame just before the stop codon. An extra 25 amino acid residues is added to Cterminal of the encoded protein after the ribosome skipping event. Three founders (#10, 42, 68) were generated. Line 10 was used for the study. (J:292649)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ddx4 (mouse) Summary of all recombinase alleles driven by Ddx4. Comparative matrix view of recombinase activities Alliance mouse Ddx4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ddx4 Mutation: 58 strains or lines available

References

• Original: J:292649 Le HT, et al., Generation of B6-Ddx4(em1(CreERT2)Utr) , a novel CreERT2 knock-in line, for germ cell lineage by CRISPR/Cas9. Genesis. 2020 Jul;58(7):e23367
• All: 1 reference(s)