Tg(CAG-EGFP,-birA*)1Fink
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,-birA*)1Fink
• Name: transgene insertion 1, Toren Fink
• MGI ID: MGI:6509971
• Synonyms: ER-BioID^HA, secretome
• Transgene: Tg(CAG-EGFP,-birA*)1Fink Location: unknown
• Alliance: Tg(CAG-EGFP,-birA*)1Fink page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct contains a CMV enhancer/chicken beta-actin (CAG) promoter, a loxP-Stop-loxP (LSL) cassette containing a green fluorescent protein sequence (EGFP) and a polyadenylation signal, followed by the ER-BioID2(HA) coding sequence. ER-BioID2(HA) is composed of an IgK signal peptide, BioID2 coding sequence (containing a mutant biotin ligase (BirA*) sequence), a hemagglutinin (HA) tag, and the endoplasmic reticulum (ER) retention signal KDEL (Lys-Asp-Glu-Leu) tetrapeptide. (J:300755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:300755 Liu J, et al., The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion. Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):e2005134118
• All: 2 reference(s)