Hprt1^{tm1(Gdf9-cas9)Jahe}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(Gdf9-cas9)Jahe}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Jason Heaney
• MGI ID: MGI:6508529
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(Gdf9-cas9)Jahe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:337011
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: B6N.Cg-A^w-J Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Endonuclease)
• Mutation: Insertion
• Hprt1^{tm1(Gdf9-cas9)Jahe} expression driven by 2 genes
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gdf9 (MGI:95692)
  mouse	Gdf9 (MGI:95692)

• Mutation details: ES cell line mEMS6131.02 (C57BL/6NTac-A(w-J)/A(w-J), Hprt1(b-m3)/Y; J:270567) was targeted with Hprt targeting vector pEMS-2001 (J:144244) harboring a 3.3 fragment of the mouse Gdf9 promoter from pGDF-9-iCre (J:93180) and S. pyogenes Cas9 from pX330-U6-Chimeric_BB-CBh-hSpCas9 (PMID: 23287718). HAT medium was used for selection and properly targeted ES cell clones were injected into C57BL/6NTac-Tyr(tm1Arte) blastocysts. Resulting chimeras were bred to C57BL/6NJ animals for germline transmission. The Gdf9 promoter drives Cas9 expression in oocytes starting at the primordial ovarian follicle stage. Maternally (oocyte) supplied S.p. Cas9 facilitates genome editing in zygotes electroporated or microinjected with a guide RNA. (J:337011)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1274 strains or lines available

Notes

ES cell = mEMS6131.02 (B6N.Cg-A^w-J Hprt^b-m3)

References

• Original: J:337011 Lanza D, et al., An Oocyte-specific Cas9-expressing mouse for germline CRISPR/Cas9-mediated genome editing. 2023
• All: 2 reference(s)