Lbhd2em1(cre/ERT2)Fuki
Endonuclease-mediated Allele Detail
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Symbol: |
Lbhd2em1(cre/ERT2)Fuki |
Name: |
LBH domain containing 2; endonuclease-mediated mutation 1, Izumi Fukunaga |
MGI ID: |
MGI:6508398 |
Synonyms: |
Lbhd2-IRES-CreERT2 |
Gene: |
Lbhd2 Location: Chr12:111373243-111378524 bp, + strand Genetic Position: Chr12, 60.98 cM
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Alliance: |
Lbhd2em1(cre/ERT2)Fuki page
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Germline Transmission: |
Earliest citation of germline transmission:
J:315131
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Parent Cell Line: |
Other (see notes) (ES Cell)
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Strain of Origin: |
C57BL/6JJcl
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Allele Type: |
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Endonuclease-mediated (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Lbhd2em1(cre/ERT2)Fuki expression driven by
1 gene
Knock-in expression driven by:
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Mutation details: Using CRISPR/cas9 technology an IRES enhancer sequence, a CreER(T2) fusion gene, and a rabbit globin polyadenylation (rGpA) termination sequence were inserted immediately downstream of the LBH domain containing 2 locus of the Lbhd2 gene on chromosome 12. The construct was electroporated into C57BL/6JJcl-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric mice were bred with C57BL/6JJcl for germline transmission.
(J:315131)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Lbhd2
(mouse)
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Original: |
J:315131 Koldaeva A, et al., Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. J Neurosci. 2021 Jul 28;41(30):6449-6467 |
All: |
1 reference(s) |
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