Tg(TcraB4,TcrbB4)#Wrh
Transgene Detail

Summary

• Symbol: Tg(TcraB4,TcrbB4)#Wrh
• Name: transgene insertion, William R Heath
• MGI ID: MGI:6508112
• Synonyms: PbT-I
• Transgene: Tg(TcraB4,TcrbB4)#Wrh Location: unknown
• Alliance: Tg(TcraB4,TcrbB4)#Wrh page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraB4,TcrbB4)#Wrh expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Transgenic Plasmodium berghei ANKA strain (PbA) MHC I-restricted (H2-K1 (H2-K^b)) TCR (PbT-I) mice were generated using the V(D)J segments of the TCRa- and b-genes of a CD8+ T cell hybridoma (B4) specific for P. berghei RPL6, a ribosomal protein expressed in various stages of the parasite life cycle. The B4 hybridoma is derived from a T cell line isolated from a blood-stage PbA-infected C57BL/6 mouse. The Tcra chain consists of V8S6, J17 and C2 gene segments and the Tcrb chain of V10 (V10S1A1), D2 and J2.2. The transgene also contains the Igh chain enhancer, the H2-K1 (H2-K^b) promoter and the polyadenylation signal sequence of the human beta-globulin gene for the Tcra segments and the Tcrb enhancer, the 2B4-derived 5' region and leader sequence and the B3-derived promoter and coding regions for the Tcrb segments. (J:274266)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:274266 Lau LS, et al., CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria. PLoS Pathog. 2014 May;10(5):e1004135
• All: 6 reference(s)