Esrp1^em1Rpc
Endonuclease-mediated Allele Detail

Summary

• Symbol: Esrp1^em1Rpc
• Name: epithelial splicing regulatory protein 1; endonuclease-mediated mutation 1, Russ P Carstens
• MGI ID: MGI:6508098
• Synonyms: Esrp1^FLAG
• Gene: Esrp1 Location: Chr4:11331933-11386783 bp, - strand Genetic Position: Chr4, 5.11 cM
• Alliance: Esrp1^em1Rpc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:293624
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: An sgRNA targeting the ATG start codon and an oligonucleotide repair template encoding two tandem copies of the FLAG epitope tag was used to generate an insertion of a FLAG tag at the beginning of the coding region. Immunohistochemical analysis showed that the epitope tagged version of the protein was expressed in epithelial cells of the developing face and palate. (J:293624)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Esrp1 Mutation: 19 strains or lines available

References

• Original: J:293624 Lee S, et al., Cleft lip and cleft palate in Esrp1 knockout mice is associated with alterations in epithelial-mesenchymal crosstalk. Development. 2020 Apr 30;147(21):dev187369
• All: 2 reference(s)