Gt(ROSA)26Sor^{tm1(CAG-Ogt,-EGFP)Xiy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Ogt,-EGFP)Xiy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Xiaoyong Yang
• MGI ID: MGI:6507417
• Synonyms: Rosa26-STOP^flox-rOGT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Ogt,-EGFP)Xiy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:283905
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into intron 1: a loxP site, a neomycin resistance gene cassette, a STOP cassette (containing SV40 poly(A) signal sequence), a second loxP site, sequence for N-terminal FLAG and HA tags, the rat GlcNAc transferase cDNA, an FRT site, IRES sequence, the EGFP fluorescent marker gene, a second FRT site, and bovine growth hormone gene poly(A) signal sequence. The inserted genes are only expressed after cre-mediated deletion of the STOP cassette. (J:283905)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:283905 Yang Y, et al., O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun. 2020 Jan 10;11(1):181
• All: 3 reference(s)