Gt(ROSA)26Sor^{tm1.1(Alb-PCSK9)Mby}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Alb-PCSK9)Mby}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Mohammad Bohlooly-Y
• MGI ID: MGI:6506458
• Synonyms: hPCSK9-KI
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Alb-PCSK9)Mby} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280156
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(Alb-PCSK9)Mby} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PCSK9 (255738)

  Pcsk9

  (MGI:2140260)

• Mutation details: A targeting vector, containing human PCSK9 cDNA under the control of a 2.34 kb mouse albumin promoter/enhancer and a floxed cassette containing cre recombinase cDNA under the control of a 698 bp testis-specific angiotensin converting enzyme promotor and neomycin phosphotransferase selection marker driven by the pol II promoter, was inserted into the endogenous locus. Flp-mediated recombination removed the floxed cre and neo cassette. (J:280156)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

Notes

PRX (C57BL/6) ES cells were used to generate mice, however the specific PRX cell line is not specified in J:280156.

References

• Original: J:280156 Carreras A, et al., In vivo genome and base editing of a human PCSK9 knock-in hypercholesterolemic mouse model. BMC Biol. 2019 Jan 15;17(1):4
• All: 1 reference(s)