Wnt1^tm1Kiv
Targeted Allele Detail

Summary

• Symbol: Wnt1^tm1Kiv
• Name: wingless-type MMTV integration site family, member 1; targeted mutation 1, Riku Kiviranta
• MGI ID: MGI:6505568
• Synonyms: Wnt1^lacZ
• Gene: Wnt1 Location: Chr15:98687738-98691711 bp, + strand Genetic Position: Chr15, 54.65 cM, cytoband F1-F3
• Alliance: Wnt1^tm1Kiv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284679
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector, PRPGS00167_B-B10 obtained from the European Conditional Mouse Mutagenesis Program (EUCOMM) inserted a cassette composed of an FRT site followed by lacZ sequence and a loxP site into the first intron between exon 1 and 2. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site, and a second loxP site. A third loxP site is inserted in exon 4. The exons 2-3 and a part of exon 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:284679)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnt1 Mutation: 29 strains or lines available

References

• Original: J:284679 Wang F, et al., Mesenchymal Cell-Derived Juxtacrine Wnt1 Signaling Regulates Osteoblast Activity and Osteoclast Differentiation. J Bone Miner Res. 2019 Jun;34(6):1129-1142
• All: 1 reference(s)