Rpe65^{tm1.1(cre/ERT2)Kser}
Targeted Allele Detail

Summary

• Symbol: Rpe65^{tm1.1(cre/ERT2)Kser}
• Name: retinal pigment epithelium 65; targeted mutation 1.1, Philip Kiser
• MGI ID: MGI:6501720
• Gene: Rpe65 Location: Chr3:159304812-159330958 bp, + strand Genetic Position: Chr3, 82.52 cM, cytoband H4
• Alliance: Rpe65^{tm1.1(cre/ERT2)Kser} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:310003
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Rpe65^{tm1.1(cre/ERT2)Kser} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Rpe65 (MGI:98001)

• Mutation details: The targeting construct was designed to fuse a P2A self-cleaving peptide sequence and a cre/ERT2 sequence in frame at the 3' of exon 14 of the retinal pigment epithelium 65 (Rpe65) gene. The cre-ERT2 fusion gene contains a Cre recombinase fused to a human estrogen receptor ligand binding domain. A frt-flanked neomycin resistance (neo) cassette was placed immediately after the non-coding exon 15. This construct was electroporated into C57BL/6 FLP-derived iTL BF1 embryonic stem (ES) cells. The Neo cassette in the targeting sequence was removed during ES clone expansion. (J:310003)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Rpe65 (mouse) Summary of all recombinase alleles driven by Rpe65. Comparative matrix view of recombinase activities Alliance mouse Rpe65 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rpe65 Mutation: 51 strains or lines available

References

• Original: J:310003 Choi EH, et al., An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight. 2021 May 10;6(9)
• All: 3 reference(s)