Pole3^em1Tbo
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pole3^em1Tbo
• Name: polymerase (DNA directed), epsilon 3 (p17 subunit); endonuclease-mediated mutation 1, Thomas Boehm
• MGI ID: MGI:6490633
• Synonyms: Pole3^m1
• Gene: Pole3 Location: Chr4:62440889-62443305 bp, - strand Genetic Position: Chr4, 33.17 cM, cytoband C1
• Alliance: Pole3^em1Tbo page

Mutation origin

• Strain of Origin: FVB

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: A deletion in the 3' end of the coding region was created using sgRNAs (targeting TACAGGCGGGAGCAGAA and ACGACCAGAACGAAGAGG) with CRISPR/Cas9 technology. The deletion removes 44 codons (132 bp), from K97 through E140, from the 3' end of the coding region, representing most of the negatively charged tail of the encoded peptide. (J:298827)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pole3 Mutation: 14 strains or lines available

References

• Original: J:298827 Siamishi I, et al., Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020 Jun 16;31(11):107756
• All: 1 reference(s)