Gt(ROSA)26Sor^{tm1(CAG-cas9)Wthg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-cas9)Wthg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Benjamin Davies
• MGI ID: MGI:6490516
• Synonyms: CAG-Cas9
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-cas9)Wthg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245962
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Endonuclease)
• Mutation: Insertion
• Mutation details: This targeted transgene overexpresses Cas9 ubiquitously by integrating a CAG-Cas9 construct at the Gt(ROSA26)Sor locus via PhiC31 integrase cassette exchange in embryonic stem cells. (J:245962)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

Notes

ES Cells = IDG26.103

References

• Original: J:245962 Cebrian-Serrano A, et al., Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models. PLoS One. 2017;12(1):e0169887
• All: 2 reference(s)