Bccip^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Bccip^{tm1d(EUCOMM)Hmgu}
• Name: BRCA2 and CDKN1A interacting protein; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6489524
• Synonyms: Bccip^-, Bccip^deltaEx5
• Gene: Bccip Location: Chr7:133311062-133322874 bp, + strand Genetic Position: Chr7, 77.3 cM, cytoband F4
• Alliance: Bccip^{tm1d(EUCOMM)Hmgu} page
• IMPC: Bccip gene page

Mutation origin

• Mutant Cell Line: HEPD0517_2_E11
• Germline Transmission: Earliest citation of germline transmission: J:297226
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 133318877 of Chromosome 7 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 5 at position 133319805. Exon 5 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:297226)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bccip Mutation: 22 strains or lines available

References

• Original: J:297226 Lu H, et al., Spontaneous Development of Hepatocellular Carcinoma and B-Cell Lymphoma in Mosaic and Heterozygous Brca2 and Cdkn1a Interacting Protein Knockout Mice. Am J Pathol. 2020 Jun;190(6):1175-1187
• All: 1 reference(s)