Tg(Cd3d-Insr*)1Mcin
Transgene Detail

Summary

• Symbol: Tg(Cd3d-Insr*)1Mcin
• Name: transgene insertion 1, Marcia McInerney
• MGI ID: MGI:6478197
• Synonyms: CD3-3FLAG-mIR/MFM
• Transgene: Tg(Cd3d-Insr*)1Mcin Location: unknown
• Alliance: Tg(Cd3d-Insr*)1Mcin page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cd3d-Insr*)1Mcin expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Insr (MGI:96575)	3XFLAG tagged

• Tg(Cd3d-Insr*)1Mcin expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cd3d (MGI:88331)

• Mutation details: The transgene insert encodes a full-length mouse insulin receptor protein cDNA containing an N-terminal 3xFLAG (DYKDDDDK) tagged alpha-subunit and full length beta-subunit as seen in the native mouse Insr gene. The endogenous signal peptide coding sequence was deleted and replaced with a pre-protrypsin (PPT) signal peptide. Thetransgene is placed downstream of the mouse Cd3d enhancer and promoter construct to elicit T-lymphocyte directed transgene over-expression. A human growth hormone (hGH) poly A signal enhances mRNA stability (J:303894)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:303894 Nandedkar-Kulkarni N, et al., Insulin Receptor-Expressing T Cells Appear in Individuals at Risk for Type 1 Diabetes and Can Move into the Pancreas in C57BL/6 Transgenic Mice. J Immunol. 2021 Apr 1;206(7):1443-1453
• All: 3 reference(s)