Gt(ROSA)26Sor^{tm1(CAG-GCaMP6,-mCherry)Shi}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GCaMP6,-mCherry)Shi}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Shionogi and Company, Ltd
• MGI ID: MGI:6477607
• Synonyms: RCL-G6-2A-mCh
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GCaMP6,-mCherry)Shi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into the locus a CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), loxP sites, neomycin resistance gene, GCaMP6 (RSET tag, chicken smooth muscle M13, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, Rat calmodulin (CaM) cDNA (aa.2-148)), sequence coding for foot-and-mouth disease virus 2A self-splicing peptide, mCherry cDNA, Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE), polyadenylation signal sequence. (J:104881, J:297280)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 5 reference(s)