Gt(ROSA)26Sor^{tm6.2(CAG-hop/EYFP)Imayo}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm6.2(CAG-hop/EYFP)Imayo}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6.2, Itaru Imayoshi
• MGI ID: MGI:6474225
• Synonyms: Gt(ROSA)26Sor^{tm1.2(CAG-eNpHR3/EYFP)Imayo}, R26-CAG-FRT-eNpHR3-EYFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm6.2(CAG-hop/EYFP)Imayo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:293482
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The transgene targeted to the locus contains a splice acceptor flanked puro cassette, CAG promoter, floxed stop cassette, FRT-flanked stop cassette, the halorhodopsin from the halophilic bacterium Natronomas pharaonis modified with an N-terminal signal peptide and a C-terminal endoplasmic reticulum export sequence fused in-frame with an enhanced yellow fluorescent protein (eNpHR3-EYFP), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability). Flp-mediated recombination removed the FRT-flanked stop cassette. Cre-mediated recombination removed the floxed stop cassette. (J:293482)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1062 strains or lines available

References

• Original: J:293482 Imayoshi I, et al., Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. Sci Rep. 2020 Feb 21;10(1):3191
• All: 1 reference(s)