Rad9a^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rad9a^{tm1b(EUCOMM)Hmgu}
• Name: RAD9 checkpoint clamp component A; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6472856
• Gene: Rad9a Location: Chr19:4245195-4251661 bp, - strand Genetic Position: Chr19, 3.89 cM, cytoband A
• Alliance: Rad9a^{tm1b(EUCOMM)Hmgu} page
• IMPC: Rad9a gene page

Mutation origin

• Mutant Cell Line: HEPD0846_6_A05
• Germline Transmission: Earliest citation of germline transmission: J:286828
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4245760 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4248128. The critical exon(s) is/are thus flanked by loxP sites. cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:286828)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad9a Mutation: 27 strains or lines available

References

• Original: J:286828 Jiang D, et al., Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. J Comp Neurol. 2020 Apr 1;528(5):729-755
• All: 1 reference(s)