Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rjsh}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rjsh}
• Name: collagen, type I, alpha 1; targeted mutation 1, Reuben J Shaw
• MGI ID: MGI:6472613
• Synonyms: Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rshaw}, iAMPK^CA
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rjsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278318
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rjsh} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRKAA1 (5562)

  Prkaa1

  (MGI:2145955)

  truncated (amino acids 1-312) for constitutively active expression

• Col1a1^{tm1(tetO-GFP,-PRKAA1*)Rjsh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The targeting construct includes a Tet-responsive element (TRE or tetO) directing expression of an eGFP reporter followed by an IRES and a truncated (amino acids 1-312) human protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1 or AMPKalpha;1) cDNA. The construct, a "Flp-In" promoter cassette (PGK-promoter-ATG-FRT) and a pCAGGS-FlpE plasmid were electroporated into modified (C57BL/6 x 129S4/SvJae)F1-derived D34 embryonic stem (ES) cells. (J:278318)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The embryonic stem cell line used is (C57BL/6 x 129S4/SvJae)F1-derived D34 that had previously been targeted with a Col1a1 "Flp-In" integration cassette (Col1a1-FRT-hygro-pA) and Gt(ROSA)26Sor^{tm2(CAG-rtTA3,-mKate2)Slowe}.

References

• Original: J:278318 Garcia D, et al., Genetic Liver-Specific AMPK Activation Protects against Diet-Induced Obesity and NAFLD. Cell Rep. 2019 Jan 2;26(1):192-208.e6
• All: 1 reference(s)