Hras^tm1.1Tumg
Targeted Allele Detail

Summary

• Symbol: Hras^tm1.1Tumg
• Name: Hras proto-oncogene, GTPase; targeted mutation 1.1, Tohoku University School of Medicine
• MGI ID: MGI:6471764
• Synonyms: Hras^G12S, Hras^{tm1.1(G12S)Tumg}
• Gene: Hras Location: Chr7:140770839-140773938 bp, - strand Genetic Position: Chr7, 86.48 cM
• Alliance: Hras^tm1.1Tumg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259739
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Single point mutation
• Mutation details: The targeting vector replaced exon 1 with a loxP site, splice acceptor, cDNA for exons 1 through 4, polyadenylaiton sequence, FRT-flanked neomycin resistance cassette, loxP site, splice acceptor, and modified exon 1 with the amino acid substitution of glycine with serine at position 12 (G12S). Cre-mediated recombination removed the cDNA allowing for expression of the mutant form. (J:259739)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hras Mutation: 29 strains or lines available

References

• Original: J:259739 Oba D, et al., Mice with an Oncogenic HRAS Mutation are Resistant to High-Fat Diet-Induced Obesity and Exhibit Impaired Hepatic Energy Homeostasis. EBioMedicine. 2018 Jan;27:138-150
• All: 2 reference(s)