Cpt1a^em1Fink
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cpt1a^em1Fink
• Name: carnitine palmitoyltransferase 1a, liver; endonuclease-mediated mutation 1, Toren Finkel
• MGI ID: MGI:6470470
• Synonyms: Cpt1a^fl
• Gene: Cpt1a Location: Chr19:3372334-3435733 bp, + strand Genetic Position: Chr19, 3.08 cM
• Alliance: Cpt1a^em1Fink page

Mutation origin

• Strain of Origin: (C57BL/6N-A^tm1Brd x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Theoriginal targeting construct is composed of an FRT site followed by a lacZ sequence and a loxP site inserted upstream of exon 4. This first loxP site was followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 4. The construct, clone HEPD0727_3_A09, was introduced into C57BL/6N-A^tm1Brd-derived JM8A3.N1 embryonic stem (ES) cells. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassette. It was then determined that the third loxP site was absent and the ES cell line was retargeted using CRISPR/Cas9 genome editing to insert a loxP site downstream of exon 4. (J:270066)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cpt1a Mutation: 54 strains or lines available

References

• Original: J:270066 Nomura M, et al., Macrophage fatty acid oxidation inhibits atherosclerosis progression. J Mol Cell Cardiol. 2019 Jan 9;127:270-276
• All: 2 reference(s)