Nanog^tm1.1Asim
Targeted Allele Detail

Summary

• Symbol: Nanog^tm1.1Asim
• Name: Nanog homeobox; targeted mutation 1.1, Antonio Simeone
• MGI ID: MGI:6468554
• Synonyms: Nanog^deltaObs3, Nanog deltaOBS5
• Gene: Nanog Location: Chr6:122684448-122691592 bp, + strand Genetic Position: Chr6, 57.8 cM, cytoband F2
• Alliance: Nanog^tm1.1Asim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238298
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Mutagenesis of Otx2 binding site (GGGATTAA into CGACAGTT) on the promoter at -144 respect to methionine 1. Recombination removed the selection cassette. (J:82809, J:238298)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nanog Mutation: 46 strains or lines available

References

• Original: J:238298 Acampora D, et al., Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast. Cell Rep. 2016 Jun 21;15(12):2651-64
• All: 2 reference(s)