Akt1^tm1.1Mjl
Targeted Allele Detail

Summary

• Symbol: Akt1^tm1.1Mjl
• Name: thymoma viral proto-oncogene 1; targeted mutation 1.1, Marjorie J Lindhurst
• MGI ID: MGI:6460342
• Synonyms: Akt1^E17K, Akt1^tm1.1Lind
• Gene: Akt1 Location: Chr12:112620260-112641266 bp, - strand Genetic Position: Chr12, 61.2 cM, cytoband F1-F2
• Alliance: Akt1^tm1.1Mjl page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:293971
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: A G to A nucleotide change at position 49 (c.49G>A) was introduced in exon 3 resulting in a glutamate to lysine substitution at amino acid 17 (p.E17K). A loxP-flanked lacZ and neomycin resistance gene fusion cassette containing a transcription termination signal was inserted upstream of exon 3. Cre-mediated recombination in the ES cells removed the lacZ/neomycin cassette resulting in the expression of the mutant protein. (J:293971)

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Akt1 Mutation: 34 strains or lines available

Notes

ES cell line = HG3 - (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1

References

• Original: J:293971 Lindhurst MJ, et al., A mouse model of Proteus syndrome. Hum Mol Genet. 2019 Sep 1;28(17):2920-2936
• All: 2 reference(s)