Kansl2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kansl2^{tm1c(EUCOMM)Wtsi}
• Name: KAT8 regulatory NSL complex subunit 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6455411
• Synonyms: Kansl2^fl
• Gene: Kansl2 Location: Chr15:98415539-98432145 bp, - strand Genetic Position: Chr15, 54.52 cM, cytoband F2
• Alliance: Kansl2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kansl2 gene page

Mutation origin

• Mutant Cell Line: EPD0240_1_A05
• Germline Transmission: Earliest citation of germline transmission: J:291094
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98426448 of Chromosome 15 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 98427781. The critical exons 5 and 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:291094)

Expression

In Mice Carrying this Mutation:	32 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kansl2 Mutation: 40 strains or lines available

References

• Original: J:291094 Sheikh BN, et al., Neural metabolic imbalance induced by MOF dysfunction triggers pericyte activation and breakdown of vasculature. Nat Cell Biol. 2020 Jul;22(7):828-841
• All: 1 reference(s)