Tg(CAG-mRuby2*,-mClover*)1Srgt
Transgene Detail

Summary

• Symbol: Tg(CAG-mRuby2*,-mClover*)1Srgt
• Name: transgene insertion 1, Sergi Regot
• MGI ID: MGI:6455404
• Synonyms: ERK-KTR^LSL
• Transgene: Tg(CAG-mRuby2*,-mClover*)1Srgt Location: unknown Genetic Position: Chr13, Syntenic
• Alliance: Tg(CAG-mRuby2*,-mClover*)1Srgt page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CMV enhancer/chicken beta-actin (CAG) promoter followed by a loxP-flanked STOP cassette, an H2B/mRuby2 fusion protein, a P2A self-cleaving peptide sequence, and an ERK-KTR/mClover fusion protein into the Gt(ROSA)26Sor locus. ERK-KTR consists of a docking site (a fragment of human ELK1, an ERK substrate), nuclear localization (NLS) and export (NES) signals, and mClover green/yellow fluorescent protein. Through nanopore sequencing and PCR verification, it was determined that the vector, along with the Rosa26 homology arms, randomly inserted into a noncoding region of chromosome 13. (J:297084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:297084 Pokrass MJ, et al., Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Dev Cell. 2020 Nov 9;55(3):328-340.e5
• All: 2 reference(s)