Gt(ROSA)26Sor^{tm3(CEBPB)Myz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CEBPB)Myz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Makoto Miyazaki
• MGI ID: MGI:6450900
• Synonyms: Rosa26-C/EBPbeta^loxtg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CEBPB)Myz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286809
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(CEBPB)Myz} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CEBPB (1051)

  Cebpb

  (MGI:88373)

  Myc-tagged

• Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert a Myc-tagged human CEBPB sequence into the Gt(ROSA)26Sor locus. R26FNF3-1F1 (1F1) embryonic stem (ES) cells were transfected with plasmids pFLSLF2-Myc human CEPBP (carrying loxP pA-puromycin-PGK-Sv40 pA (in reverse orientation)-loxP-Myc-CEBPB-WPRE-BGH pA) and pCAG-Flpe (expressing FLP recombinase). The 1F1 ES cell line used to generate the mutation was created by targeting Gt(ROSA)26Sor with an FRT- and F3-flanked PGK-neomycin selection cassette by homologous recombination in (C57BL/6J x 129X1/SvJ)F1-derived EC7.1 ES cells. (J:286809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 956 strains or lines available

References

• Original: J:286809 Kohno S, et al., An N-terminal-truncated isoform of FAM134B (FAM134B-2) regulates starvation-induced hepatic selective ER-phagy. Life Sci Alliance. 2019 Jun;2(3)
• All: 1 reference(s)