Capn15^{tm2c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Capn15^{tm2c(EUCOMM)Hmgu}
• Name: calpain 15; targeted mutation 2c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6450514
• Synonyms: Capn15 cKO
• Gene: Capn15 Location: Chr17:26177338-26204753 bp, - strand Genetic Position: Chr17, 13.04 cM, cytoband A3
• Alliance: Capn15^{tm2c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292541
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25963805 upstream of exon 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 25962456. Flp-mediated recombination removed the FRT-flanked lacZ/neo cassette leaving exons 3-5 floxed. (J:292541)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Capn15 Mutation: 28 strains or lines available

References

• Original: J:292541 Zha C, et al., MRI of Capn15 knockout mice and analysis of Capn15 distribution reveal possible roles in brain development and plasticity. bioRxiv. 2020
• All: 4 reference(s)