H6pd^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: H6pd^{tm1c(EUCOMM)Wtsi}
• Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6449815
• Synonyms: H6PDH^fl
• Gene: H6pd Location: Chr4:150063932-150093480 bp, - strand Genetic Position: Chr4, 80.65 cM
• Alliance: H6pd^{tm1c(EUCOMM)Wtsi} page
• IMPC: H6pd gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292236
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_st0 cassette was inserted at position 150078658 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 150079496. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:292236)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H6pd Mutation: 62 strains or lines available

References

• Original: J:292236 Wang J, et al., Lack of adipose-specific hexose-6-phosphate dehydrogenase causes inactivation of adipose glucocorticoids and improves metabolic phenotype in mice. Clin Sci (Lond). 2019 Nov 15;133(21):2189-2202
• All: 1 reference(s)