Pramex1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pramex1^{tm1c(KOMP)Wtsi}
• Name: PRAME like, X-linked 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6449711
• Synonyms: Prame^fl, Prame^{tm1c(KOMP)Wtsi}
• Gene: Pramex1 Location: ChrX:134513751-134528454 bp, - strand Genetic Position: ChrX, 57.26 cM, cytoband F1
• Alliance: Pramex1^{tm1c(KOMP)Wtsi} page
• IMPC: Pramex1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:290700
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 134514970 of Chromosome X upstream of the critical exons (exons 8 and 9) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 134517047. Exons 8 and 9 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 8 and 9. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:290700)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pramex1 Mutation: 8 strains or lines available

References

• Original: J:290700 Lu C, et al., Deletion of the mouse X-linked Prame gene causes germ cell reduction in spermatogenesis. Mol Reprod Dev. 2020 Feb 3
• All: 1 reference(s)