Gt(ROSA)26Sor^{tm3(CAG-sfGFP*)Xwy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-sfGFP*)Xwy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, X William Yang
• MGI ID: MGI:6441965
• Synonyms: MORF3
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-sfGFP*)Xwy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292704
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed by using the Ai9 plasmid and replacing the tdTomato reporter with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized C)22(-repeat MORF switch, and a farnesylated tamdem superfolder GFP fluorescent reporter with several V5 epitopes (tdsmFP-V5). Superfolder (sfGFP) is a hyperstable GFP variant that accepts large insertions into its loops while retaining folding and fluorescence. To create tdsmFP-V5, sfGFP was altered to embed several V5 epitopes (V5 is a 14 amino acid simian virus 5 epitope) in the GFP scaffold - 3 epitopes at the N terminus, 4 epitopes into the internal 172-173 loop and 3 epitopes at the C terminus. The tandem dimer of smFP-V5 contains a total of 20 V5 epitope tags. The inclusion of the V5 epitopes creates a highly antigenic spaghetti monster fluorescent protein (smGFP) that contains numerous copies of peptide epitopes and simultaneously binds IgG antibodies at each location. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. The final vector contains from 5' to 3' a CAG promoter, an FRT site, a loxP-flanked STOP cassette, the tdsmFP-V5 construct (see above), followed by a WPRE, polyA, attB-PGK-FRT-neo-pA-attP inserted in to the Gt(ROSA)26Sor locus. (J:292704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:292704 Veldman MB, et al., Brainwide Genetic Sparse Cell Labeling to Illuminate the Morphology of Neurons and Glia with Cre-Dependent MORF Mice. Neuron. 2020 Aug 5
• All: 4 reference(s)