Gt(ROSA)26Sor^{tm2(CAG-mNeonGreen)Xwy}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-mNeonGreen)Xwy}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, X William Yang
• MGI ID: MGI:6441960
• Synonyms: MORF1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-mNeonGreen)Xwy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292704
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector was designed by using the Ai9 plasmid and replacing the tdTomato reporter with a Kozak consensus start site (for strong initiation of translation), two Myc-tags, an optimized C)22(-repeat MORF switch, and a farnesylated mNeonGreen fluorescent reporter (mNG-F). mNeonGreen is one of the brightest green/yellow fluorescent proteins and is derived from Branchiostoma lanceolatum. The farnesylation sequence (CAAX domain from Ras) targets the reporter to membranous structures including neuronal dendrites and axons. The final vector contains from 5' to 3' a CAG promoter, an FRT site, a loxP-flanked STOP cassette, the mNeonGreen construct (see above), followed by a WPRE, polyA, attB-PGK-FRT-neo-pA-attP inserted in to the Gt(ROSA)26Sor locus. (J:292704)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:292704 Veldman MB, et al., Brainwide Genetic Sparse Cell Labeling to Illuminate the Morphology of Neurons and Glia with Cre-Dependent MORF Mice. Neuron. 2020 Aug 5
• All: 1 reference(s)