B4galt5^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: B4galt5^em1(IMPC)Tcp
• Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6435667
• Gene: B4galt5 Location: Chr2:167140364-167191103 bp, - strand Genetic Position: Chr2, 87.22 cM, cytoband H3
• Alliance: B4galt5^em1(IMPC)Tcp page
• IMPC: B4galt5 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR1529 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of GAGACCTGCTTCTGCCGAGG targeting the 5' side and AGGTCTCTAGCTAGACGCTT targeting the 3' side of a critical region. This resulted in 596-bp deletion, Chr2:167307195 to 167307790 (GRCm38). (J:265051)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any B4galt5 Mutation: 25 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 3 reference(s)