Shmt1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Shmt1^{tm1c(KOMP)Wtsi}
• Name: serine hydroxymethyltransferase 1 (soluble); targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6416316
• Gene: Shmt1 Location: Chr11:60679723-60702062 bp, - strand Genetic Position: Chr11, 37.84 cM
• Alliance: Shmt1^{tm1c(KOMP)Wtsi} page
• IMPC: Shmt1 gene page

Mutation origin

• Mutant Cell Line: EPD0551_3_A06
• Germline Transmission: Earliest citation of germline transmission: J:169366
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 60689189 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 60689952. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:169366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Shmt1 Mutation: 44 strains or lines available

References

• Original: J:169366 MouseBook^TM, Information obtained from MouseBook^TM, Medical Research Council Mammalian Genetics Unit, Harwell, UK. Unpublished. 2005-2013
• All: 1 reference(s)