Ttk^tm1.1Kops
Targeted Allele Detail

Summary

• Symbol: Ttk^tm1.1Kops
• Name: Ttk protein kinase; targeted mutation 1.1, Geert Kops
• MGI ID: MGI:6404032
• Synonyms: CiMKi^TA
• Gene: Ttk Location: Chr9:83716742-83754442 bp, + strand Genetic Position: Chr9, 45.61 cM
• Alliance: Ttk^tm1.1Kops page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:286421
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector was designed to insert a loxP-flanked cassette containing cDNA encoding exons 17-22 from the gene downstream of the endogenous exon 16. The 3' loxP site was preceded by a stop codon and polyadenylation sequence. Downstream of this cassette, endogenous exon 17 was modified to harbor an ACA to GCA mutation at codon 649, resulting in a threonine to alanine mutation (T649A) similar to that found in human cell lines with a mild chromosomal instability (CIN) phenotype. An FRT-flanked puromycin cassette was also included and then removed by Flp-mediated recombination. (J:286421)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ttk Mutation: 53 strains or lines available

References

• Original: J:286421 Hoevenaar WHM, et al., Degree and site of chromosomal instability define its oncogenic potential. Nat Commun. 2020 Mar 20;11(1):1501
• All: 1 reference(s)