Tsen54^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tsen54^{tm1b(EUCOMM)Wtsi}
• Name: tRNA splicing endonuclease subunit 54; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6402621
• Synonyms: Tsen54^tm1b
• Gene: Tsen54 Location: Chr11:115705550-115713920 bp, + strand Genetic Position: Chr11, 80.91 cM, cytoband E2
• Alliance: Tsen54^{tm1b(EUCOMM)Wtsi} page
• IMPC: Tsen54 gene page

Mutation origin

• Mutant Cell Line: EPD0326_5_H07
• Germline Transmission: Earliest citation of germline transmission: J:261024
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 115707390 of Chromosome 11 upstream of exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 115708216. Exon 6 is thus flanked by loxP sites. Subsequent cre-mediated recombination resulted in this reporter knockout allele. (J:261024)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tsen54 Mutation: 34 strains or lines available

References

• Original: J:261024 Ermakova O, et al., Three-dimensional microCT imaging of murine embryonic development from immediate post-implantation to organogenesis: application for phenotyping analysis of early embryonic lethality in mutant animals. Mamm Genome. 2018 Apr;29(3-4):245-259
• All: 2 reference(s)