Sdr16c6^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sdr16c6^{tm1d(KOMP)Wtsi}
• Name: short chain dehydrogenase/reductase family 16C, member 6; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6400337
• Gene: Sdr16c6 Location: Chr4:4055926-4077514 bp, - strand Genetic Position: Chr4, 2.27 cM
• Alliance: Sdr16c6^{tm1d(KOMP)Wtsi} page
• IMPC: Sdr16c6 gene page

Mutation origin

• Mutant Cell Line: EPD0583_3_F03
• Germline Transmission: Earliest citation of germline transmission: J:283063
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4062948 of Chromosome 4 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 4062948. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:283063)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdr16c6 Mutation: 16 strains or lines available

References

• Original: J:283063 Wu L, et al., Mice lacking the epidermal retinol dehydrogenases SDR16C5 and SDR16C6 display accelerated hair growth and enlarged meibomian glands. J Biol Chem. 2019 Nov 8;294(45):17060-17074
• All: 1 reference(s)