Col1a1^{tm1(RHOA*Y42C)Abass}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(RHOA*Y42C)Abass}
• Name: collagen, type I, alpha 1; targeted mutation 1, Adam Bass
• MGI ID: MGI:6393676
• Synonyms: LSL-RHOA^Y42C
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(RHOA*Y42C)Abass} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:284071
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6 and 129

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Nucleotide substitutions
• Col1a1^{tm1(RHOA*Y42C)Abass} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RHOA (387)

  Rhoa	(MGI:1096342)

  contains Y42C mutation

• Mutation details: A loxP-Stop-loxP-Human RHOA cDNA cassette, modified with a Y42C amino acid mutation (nucleotides TAT to TGT) in RHOA, was introduced to an intergenic region downstream of the mouse Col1a1 gene via Flp recombinase-mediated homologous recombination. (J:284071)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

The allele was made in MESC10 ES cells that carry an FRT-PGK-neomycin-FRT (Flp in) site downstream of the mouse Col1a1 gene.

References

• Original: J:284071 Zhang H, et al., Gain-of-Function RHOA Mutations Promote Focal Adhesion Kinase Activation and Dependency in Diffuse Gastric Cancer. Cancer Discov. 2020 Feb;10(2):288-305
• All: 1 reference(s)