Gt(ROSA)26Sor^{tm1(CAG-CD9/GFP)Dmfel}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-CD9/GFP)Dmfel}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David Feliciano
• MGI ID: MGI:6392825
• Synonyms: TIGER
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-CD9/GFP)Dmfel} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:275836
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S1/SvImJ)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-CD9/GFP)Dmfel} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD9 (928)

  Cd9	(MGI:88348)

  fused to turboGFP with a carboxy terminal His tag

• Mutation details: A targeting vector containing the CAG promoter, a floxed STOP cassette followed by a carboxy terminal His-tagged CD9/GFP cassette, and a NeoR selection cassette was inserted into the Gt(ROSA)26Sor locus. CD9/GFP is human CD9 containing a carboxy terminal His-tag and fused to copepod green fluorescent protein (CopGFP or Turbo-GFP from Pontellina plumata). (J:275836)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

Notes

S1B6A embryonic stem cells

References

• Original: J:275836 Neckles VN, et al., A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles. Sci Rep. 2019 Feb 28;9(1):3094
• All: 1 reference(s)