Col7a1^em#Jtol
Endonuclease-mediated Allele Detail

Summary

• Symbol: Col7a1^em#Jtol
• Name: collagen, type VII, alpha 1; endonuclease-mediated mutation, Jakub Tolar
• MGI ID: MGI:6390909
• Synonyms: Col7a1^-
• Gene: Col7a1 Location: Chr9:108782654-108813943 bp, + strand Genetic Position: Chr9, 59.63 cM
• Alliance: Col7a1^em#Jtol page

Mutation origin

• Strain of Origin: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology using two gRNAs 5'-CAGGATGAGGCTGCGACTCG-3' and 5'-TGATGGGAGCGCCGGAAGTG-3' targeted insertions and deletions within the first exon to produce null alleles. The pound symbol (#) is used when no line is specified or lines are pooled. (J:280214)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col7a1 Mutation: 106 strains or lines available

References

• Original: J:280214 Webber BR, et al., Rapid generation of Col7a1(-/-) mouse model of recessive dystrophic epidermolysis bullosa and partial rescue via immunosuppressive dermal mesenchymal stem cells. Lab Invest. 2017 Oct;97(10):1218-1224
• All: 1 reference(s)