Gt(ROSA)26Sor^{tm7(CAG-dre/ERT2)Bzsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm7(CAG-dre/ERT2)Bzsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 7, Bin Zhou
• MGI ID: MGI:6388407
• Synonyms: R26-LDLZ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm7(CAG-dre/ERT2)Bzsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278877
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm7(CAG-dre/ERT2)Bzsh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: A Rosa-CAG targeting vector was designed to insert cassette containing (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), loxP-site, Dre recombinase fused with a mutant form of estrogen receptor hormon-binding domaine C (ERT2), STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), second loxP-site, ZsGreen sequence (an enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), WPRE (woodchuck hepatitis virus posttranscriptional regulatory element)-polyA, and Frt flanked neomicyne selection cassette (CAG-loxp-DreERT2-stop-loxp-ZsGreen-polyA-Frt-Neo-Frt) into the Rosa26 locus. (J:278877)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1058 strains or lines available

References

• Original: J:278877 Li Y, et al., Genetic Tracing Identifies Early Segregation of the Cardiomyocyte and Nonmyocyte Lineages. Circ Res. 2019 Jul 19;125(3):343-355
• All: 5 reference(s)