Gt(ROSA)26Sor^{tm1(CAG-ZsGreen,-tdTomato)Bzsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ZsGreen,-tdTomato)Bzsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bin Zhou
• MGI ID: MGI:6386765
• Synonyms: interleaved reporter 1, IR1
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ZsGreen,-tdTomato)Bzsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278073
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A Rosa-CAG targeting vector was designed to insert cassette containing (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), loxP-site, rox-site, STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), second loxP-site, ZsGreen sequence (an enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), second STOP (adapted from the polyA sequence between mT (membrane-targeted tdTomato), second rox-site, tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), WPRE (woodchuck hepatitis virus posttranscriptional regulatory element)-polyA, and Frt flanked neomicyne selection cassette (CAG-loxP-rox-Stop-loxP-ZsGreen-Stop-rox-tdTomato-polyA-Frt-Neo-Frt) into the Rosa26 locus. (J:278073)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:278073 He L, et al., Enhancing the precision of genetic lineage tracing using dual recombinases. Nat Med. 2017 Dec;23(12):1488-1498
• All: 6 reference(s)