Flt3^em1Doi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Flt3^em1Doi
• Name: FMS-like tyrosine kinase 3; endonuclease-mediated mutation 1, Christophe Benoist and Diane Mathis
• MGI ID: MGI:6386252
• Synonyms: Flt3^itagBFP2
• Gene: Flt3 Location: Chr5:147267551-147337299 bp, - strand Genetic Position: Chr5, 86.88 cM, cytoband G3
• Alliance: Flt3^em1Doi page

Mutation origin

• Strain of Origin: NOD

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 genome editing is used to introduce a 5' HA tag, a TagBFP2 (a blue variant of GFP), IRES and a 3' HA tag into exon 24. (J:253628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Flt3 Mutation: 94 strains or lines available

References

• Original: J:253628 Mohan JF, et al., Imaging the emergence and natural progression of spontaneous autoimmune diabetes. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):E7776-E7785
• All: 1 reference(s)