Tnni3k^em1Hsv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnni3k^em1Hsv
• Name: TNNI3 interacting kinase; endonuclease-mediated mutation 1, Henry M Sucov
• MGI ID: MGI:6384879
• Synonyms: K489R
• Gene: Tnni3k Location: Chr3:154491928-154761044 bp, - strand Genetic Position: Chr3, 79.32 cM
• Alliance: Tnni3k^em1Hsv page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPER/Cas9 genome editing is used to change the lysine codon at position 489 to arginine (AAA to AGA) in exon 15 of the gene. This mutation results in a premature stop codon homologous to one found in exon 16, early in the encoding portion of the kinase domain, in the naked mole-rat species Heterocephalus glaber. Two G-to-T substitutions were also introduced in order to eliminate the Cas9 PAM sequence to prevent retargeting. (J:280429)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnni3k Mutation: 44 strains or lines available

References

• Original: J:280429 Gan P, et al., Tnni3k alleles influence ventricular mononuclear diploid cardiomyocyte frequency. PLoS Genet. 2019 Oct;15(10):e1008354
• All: 2 reference(s)