Dmtn^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Dmtn^{tm1c(KOMP)Mbp}
• Name: dematin actin binding protein; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:6384825
• Synonyms: Epb4.9^fl
• Gene: Dmtn Location: Chr14:70839624-70873488 bp, - strand Genetic Position: Chr14, 36.32 cM
• Alliance: Dmtn^{tm1c(KOMP)Mbp} page
• IMPC: Dmtn gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235867
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at a position on Chromosome 14 downstream of exon 4. The cassette is composed of a FRT site followed by En2SA (mouse engrailed 2 gene splice acceptor), internal ribosome entry site (IRES) fused to a beta-galactosidase (lacZ) gene, and a polyadenylation site (SV40 polyA). This was followed by a loxP-flanked cassette containing a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 5 through 8 floxed. (J:235867)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dmtn Mutation: 160 strains or lines available

Notes

Personal communication from the investigator, ES cells for Dmtn (Epb4.9) were acquired from KOMP.

References

• Original: J:235867 Lu Y, et al., Gene disruption of dematin causes precipitous loss of erythrocyte membrane stability and severe hemolytic anemia. Blood. 2016 Jul 7;128(1):93-103
• All: 2 reference(s)