Mir290a^{em1.1(Snrpn-tdTomato)Jae}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mir290a^{em1.1(Snrpn-tdTomato)Jae}
• Name: microRNA 290a; endonuclease-mediated mutation 1.1, Rudolf Jaenisch
• MGI ID: MGI:6383984
• Synonyms: MIR290-129^{SE-RGM-tdTomato}, Mir290-SE-T
• Gene: Mir290a Location: Chr7:3267300-3267382 bp, + strand Genetic Position: Chr7, 1.88 cM
• Alliance: Mir290a^{em1.1(Snrpn-tdTomato)Jae} page

Mutation origin

• Strain of Origin: 129

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to target the super enhancer (SE) sequences ~15 kb proximal of the microRNA 290a locus on chromosome 7, as well as Snrpn::tdTomato::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter, the tdTomato fluorescent protein, a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES c ells with the Snrpn::tdTomato::WPRE::BHGpA sequences inserted into the Mir290a SE on the 129S1 chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro cassette. (J:282716)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir290a Mutation: 5 strains or lines available

References

• Original: J:282716 Song Y, et al., Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Sep 5;75(5):905-920.e6
• All: 1 reference(s)