Sox2^{em1.1(Snrpn-tdTomato)Jae}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sox2^{em1.1(Snrpn-tdTomato)Jae}
• Name: SRY (sex determining region Y)-box 2; endonuclease-mediated mutation 1.1, Rudolf Jaenisch
• MGI ID: MGI:6383980
• Synonyms: Sox2-129^{SE-RGM-tdTomato}, SOX2-SE-T
• Gene: Sox2 Location: Chr3:34704554-34706610 bp, + strand Genetic Position: Chr3, 16.93 cM, cytoband A2-B
• Alliance: Sox2^{em1.1(Snrpn-tdTomato)Jae} page

Mutation origin

• Strain of Origin: 129

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing is used to target the super enhancer (SE) sequences ~100 kb distal of the SRY (sex determining region Y)-box 2 locus (Sox2) on chromosome 3, as well as Snrpn::tdTomato::WPRE::BHGpA::loxP-PGK-Puro-loxP sequences (encoding the minimal Snrpn promoter, the tdTomato fluorescent protein, a WPRE sequence, a BGH polyadenylation sequence and a loxP-flanked PGK-Puro selection cassette). Junction PCR-SNP analyses identified ES cells with the S2T sequences (Snrpn::tdTomato::WPRE::BHGpA) inserted into the Sox2 SE on the 129 chromosome. Those ES cells were transfected with a Cre recombinase-expressing plasmid to remove the PGK-Puro, (J:282716)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sox2 Mutation: 56 strains or lines available

References

• Original: J:282716 Song Y, et al., Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Mol Cell. 2019 Sep 5;75(5):905-920.e6
• All: 1 reference(s)