Gt(ROSA)26Sor^{em1.1(CAG-RFP*)Jrt}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1.1(CAG-RFP*)Jrt}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1.1, Janet Rossant
• MGI ID: MGI:6383974
• Synonyms: miRFP703, R26-CAG-H2B-miRFP703
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1.1(CAG-RFP*)Jrt} page

Mutation origin

• Strain of Origin: CD-1

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease-mediated genome editing was used to introduce a CAG-H2B-miRFP703 (nuclear localized) vector into the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette. The STOP cassette was removed by an in vitro cre reaction on the plasmid. miRFP703 is derived from miRFP670, which is itself derived from RpBphP1, a bacterial phytochrome photoreceptor, Rhodopseudomonas palustris. miRFP703 includes the following mutations: D16S/L17H/C20S/R22H/P43H/I47V/D106C/P127S/A155V/E179L/R184C/D201T/I202V/R208Q/E211V/S222T/I253C/Y257F/N260D/V268A/A282V/H292R/E300K/L301R/A305R/S20C/T201L/C253I. (J:266345)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:266345 Gu B, et al., Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nat Biotechnol. 2018 Aug;36(7):632-637
• All: 2 reference(s)