Trpc5^tm1.1Mfre
Targeted Allele Detail

Summary

• Symbol: Trpc5^tm1.1Mfre
• Name: transient receptor potential cation channel, subfamily C, member 5; targeted mutation 1.1, Marc Freichel
• MGI ID: MGI:6383454
• Synonyms: Trpc5-E4fx, Trpc5^L3F1
• Gene: Trpc5 Location: ChrX:143164667-143471176 bp, - strand Genetic Position: ChrX, 65.49 cM, cytoband F2
• Alliance: Trpc5^tm1.1Mfre page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:281818
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 4. A floxed cassette with a splice acceptor, IRES, and GFP reporter gene were inserted downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette previously located within the reporter cassette. Immunohistochemistry confirms the reduction in transcript expression in ARC neurons. (J:281818, J:277600)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpc5 Mutation: 19 strains or lines available

References

• Original: J:281818 Xue T, et al., Melanopsin signalling in mammalian iris and retina. Nature. 2011 Nov 2;479(7371):67-73
• All: 2 reference(s)