Zfp148^tm1.2Bchac
Targeted Allele Detail

Summary

• Symbol: Zfp148^tm1.2Bchac
• Name: zinc finger protein 148; targeted mutation 1.2, Alan Cantor
• MGI ID: MGI:6383008
• Gene: Zfp148 Location: Chr16:33201145-33324273 bp, + strand Genetic Position: Chr16, 23.93 cM
• Alliance: Zfp148^tm1.2Bchac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282446
• Parent Cell Line: CJ9 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector contains a loxP site placed in intron 5 and an FRT::neomycin-delta thymidine kinase (Neo-DTK)::FRT::loxP cassette placed in intron 7. Flp-mediated recombination removed the FRT-flanked neomycin cassette. Cre-mediated recombination removed exons 6 and 7. The deletion creates a premature translation termination codon in exon 8 and deletes 80% of the coding region including the entire zinc finger DNA binding domain. (J:282446)

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp148 Mutation: 112 strains or lines available

References

• Original: J:282446 Woo AJ, et al., Zfp281 (ZBP-99) plays a functionally redundant role with Zfp148 (ZBP-89) during erythroid development. Blood Adv. 2019 Aug 27;3(16):2499-2511
• All: 1 reference(s)