Gtf2h3^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gtf2h3^em1(IMPC)J
• Name: general transcription factor IIH, polypeptide 3; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6382787
• Gene: Gtf2h3 Location: Chr5:124717211-124735743 bp, + strand Genetic Position: Chr5, 63.67 cM
• Alliance: Gtf2h3^em1(IMPC)J page
• IMPC: Gtf2h3 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CCCATGGCAAAGAAGCTGAG and ATACACCAATTCCCACCACA, which resulted in a 695 bp deletion beginning at Chromosome 5 position 124,583,657 bp and ending after 124,584,351 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001266779 and ENSMUSE00001246016 (exons 3 and 4) and 421 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 31 and early truncation 25 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gtf2h3 Mutation: 52 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)